Transgene expression, but not gene delivery, is improved by adhesion-assisted lipofection of hematopoietic cells
نویسندگان
چکیده
منابع مشابه
CD93 is Selectively Expressed on Human Myeloma Cells but Not on B Lymphocytes
Background: CD93 has originally been known as a C1q receptor, and many studies have demonstrated that CD93 is expressed on hematopoietic stem cells, B cell progenitors, myeloid and monocytic cells. Moreover, CD93 is shown to be expressed on long-lived plasma cells, and CD93 deficient-mice display an impairment in plasma cell development. Objective: To investiga...
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It has recently been reported that in a xenotransplantation model of human cells injected into nonobese diabetic–severe combined immunodeficient mice, direct intratibial injection of stem cells improves engraftment.1,2 However, the complexities of using this approach clinically are substantial. We sought to determine whether in a syngeneic mouse model the delivery of donor primitive hematopoiet...
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Gene delivery to neural cells is central to the development of transplantation therapies for neurological diseases. In this study, we used a baculovirus derived from the domesticated silk moth, Bombyx mori, as vector for transducing a human cell line (HEK293) and primary cultures of rat Schwann cells. Under optimal conditions of infection with a recombinant baculovirus containing the reporter g...
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ژورنال
عنوان ژورنال: Gene Therapy
سال: 1999
ISSN: 0969-7128,1476-5462
DOI: 10.1038/sj.gt.3300896